This mouse Factor X total assay is for the quantitative determination of total Factor X and Xa in biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.

Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with Streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total Factor X in the sample.

More Details:

• Detection Target: Factor X
• Reactivity: Mouse
• Range: 2.5-500 ng/ml
• Sensitivity: 0.146 ng/ml

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Please note – this product replaces our old product number IMFXKT-TOT (same product, new number).