Mouse uPA activity assay is intended for the quantitative determination of active plasminogen activator in mouse plasma. For research use only. Strip well format. Reagents for up to 96 tests.

Functionally active uPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.

More Details:

• Detection Target: uPA
• Reactivity: Mouse
• Range: 0.025-10 ng/mll
• Sensitivity: 0.02 ng/ml

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Please note – this product replaces our old product number IMUPAKT (same product, new number).