Human uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in human plasma and urine. For research use only. Strip well format. Reagents for up to 96 tests.

Functionally active uPA present in samples will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.

More Details:

• Detection Target: uPA
• Reactivity: Human
• Range: 0.1-50 ng/ml
• Sensitivity: 0.013 ng/ml

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Please note – this product replaces our old product number IHPAKT (same product, new number).