This human immunoglobulin E (IgE) antigen assay is intended for the quantitative determination of total human IgE antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.

Human IgE will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgE antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgE. Color development is proportional to the concentration of IgE in the samples.

More Details:

• Detection Target: IgE
• Reactivity: Human
• Range: 0.5-500 ng/ml
• Sensitivity: 0.38 ng/ml

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Please note – this product replaces our old product number IHUIGEKT (same product, new number).